Comprehensive analysis of the isozyme composition of laccase derived from Japanese lacquer tree, Toxicodendron vernicifluum

Abstract We performed an analysis using isoelectric focusing to comprehensively clarify the isozyme composition of laccase derived from Japanese lacquer tree, Toxicodendron vernicifluum . When water extracts acetone powder obtained were subjected focusing, five bands within pI 7.35–9.30 and nine 3.50–5.25 detected Coomassie staining. Similarly, activity staining guaiacol showed three 3.50–4.25. However, gallic acid remarkable 3.50–5.85, whereas was very weak 7.35–9.30. fractionated into fractions containing 3.50–5.85 by SP-Sepharose column chromatography, former had a blue color latter yellow color. The measured for each in buffer solution pH range 2.5–8.0. syringaldazine, guaiacol, 2,6-dimethoxyphenol used as substrates, fraction considerably higher than 5.5–7.5. 3-methylcatechol 4-methylcatechol 4.5–6.5, 7.0–8.0. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate, both maximum at optimum 3.0–4.0. Conventionally, research on lacquer, non-blue corresponding lower 6 has been removed during purification process thus not analyzed. Our results indicated that present components it may play role urushiol polymerization with previously reported laccase.